Uridine CAS 58-96-8 Purity ≥99.0% (HPLC) Content 98.0%-102.0% (UV) Factory High Purity

4 Uridine (CAS: 58-96-8) Method of Analysis
4.1 Appearance
The appearance of Uridine should be white or or almost white crystalline powder, by visual.
4.2 Loss on drying: ≤0.50%
According to the "Pharmacopoeia of the People's Republic of China, second edition, 2005 " Appendix 53Ⅷ L loss on drying determination method.
4.3 Specific Rotation [a]20/D +6.0°~+10.0° (2% aqueous solution)
4.4 Melting point: 162.0~171.0℃
According to the "Pharmacopoeia of the People's Republic of China, second edition, 2005 " Appendix 37 VI C melting point determination method.
4.5 Residue on ignition: ≤0.10%
According to the "Pharmacopoeia of the People's Republic of China, second edition, 2005" Appendix 54 Ⅷ N Residue on ignition inspection method.
4.6 Heavy metals: ≤10ppm
According to the "Pharmacopoeia of the People's Republic of China, second edition, 2005" Appendix ⅧH heavy metal test method.
4.7 Content (UV): 98.0%~102.0%
Sample preparation: Precision weighing and baking to constant weight sample 100.0mg (accurate to 0.0001g), with 0.1mol/L hydrochloric acid dissolved into 200ml volumetric bottle constant volume, absorb 2ml to 100ml volumetric bottle constant volume, shake well to become a solution containing about 10ug per ml, measuring wavelength of 262nm.
Uridine content %=A262×10000×M×100%/ε×W
Where A262 is the absorbance of the sample measured at 262nm
M is the molecular weight of the sample, 244.2
ε is the maximum extinction coefficient of the sample, 10100
W is the sample weight, mg
4.8 Purity (HPLC) : ≥99.0%
Determination of Uridine by high performance liquid chromatography (HPLC) according to Appendix 28 VD of Pharmacopoeia of the People's Republic of China, second edition, 2005.
Mobile phase preparation:
Acetonitrile was [(Na2HPO4 1.884g+NaH2PO4 0.726g) /L] = 3:97 as mobile phase.
The chromatographic column was C18 column 4.6 ×250mm.
Appropriate amount of samples were taken, dissolved and shaken with mobile phase, and samples were injected. The detection wavelength was 260nm.
According to the area normalization method, the measured content shall not be less than 99.0%.